Spectral characterization ofDictyostelium autofluorescence
نویسندگان
چکیده
منابع مشابه
Spectral characterization of Dictyostelium autofluorescence.
Dictyostelium discoideum is used extensively as a model organism for the study of chemotaxis. In recent years, an increasing number of studies of Dictyostelium chemotaxis have made use of fluorescence-based techniques. One of the major factors that can interfere with the application of these techniques in cells is the cellular autofluorescence. In this study, the spectral properties of Dictyost...
متن کاملFundus Autofluorescence and Spectral Domain OCT in Central Serous Chorioretinopathy
Background. To describe the standard autofluorescence (FAF), the near infrared autofluorescence (NIA) and optical coherence tomography (OCT) patterns in central serous chorioretinopathy, correlating them with fluorescein angiography. Methods. Cross-sectional observational study, in which patients with at least seven months of CSC underwent ophthalmologic examination, fundus photography, FAF, NI...
متن کاملSuppression of Autofluorescence based on Fuzzy Classification by Spectral Angles
Background fluorescence, also known as autofluorescence, and cross-talk are two problems in fluorescence microscopy that stem from similar phenomena. When biological specimens are imaged, the detected signal often contains contributions from fluorescence originating from sources other than the imaged fluorophore. This fluorescence could either come from the specimen itself (autofluorescence), o...
متن کاملLabel-free microscopy: spectral imaging of multiphoton-excited cellular autofluorescence
A. Holloschi, HM. Kuhn, C. Müller, M. Worf, M. Rauen, T. Röder, W. Kessler, J. Mollenhauer and P. Kioschis 1 University of Applied Sciences Mannheim, Institute of Molecular and Cell Biology and Institute of Chemical Process Engineering, Mannheim, Germany 2 Steinbeis-Hochschule Berlin, Steinbeis-Insitut Multivariate Datenanalyse, Berlin, Germany 3 University of Southern Denmark, Lundbeckfonden C...
متن کاملAutofluorescence characterization of advanced glycation end products of hemoglobin.
This article describes the analysis of autofluorescence of advanced glycation end products of hemoglobin (Hb-AGE). Formed as a result of slow, spontaneous and non-enzymatic glycation reactions, Hb-AGE possesses a characteristic autofluorescence at 308/345 nm (lambda(ex)/lambda(em)). Even in the presence of heme as a quenching molecule, the surface presence of the glycated adduct gave rise to au...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy Research and Technique
سال: 2006
ISSN: 1059-910X,1097-0029
DOI: 10.1002/jemt.20282